Novel florfenicol and chloramphenicol resistance gene discovered in Alaskan soil by using functional metagenomics

thumbnail of Lang-flor-res-alaska-metagen-AEM-2010 Lang KS, Anderson JM, Schwarz S, Williamson L, Handelsman J, Singer RS
Applied and Environmental Microbiology 76:5321-5326

Functional metagenomics was used to search for florfenicol resistance genes in libraries of cloned DNA isolated from Alaskan soil. A gene that mediated reduced susceptibility to florfenicol was identified and designated pexA. The predicted PexA protein showed a structure similar to that of efflux pumps of the major facilitator superfamily.

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