Novel florfenicol and chloramphenicol resistance gene discovered in Alaskan soil by using functional metagenomicsLang KS, Anderson JM, Schwarz S, Williamson L, Handelsman J, Singer RS
Applied and Environmental Microbiology 76:5321-5326
Functional metagenomics was used to search for florfenicol resistance genes in libraries of cloned DNA isolated from Alaskan soil. A gene that mediated reduced susceptibility to florfenicol was identified and designated pexA. The predicted PexA protein showed a structure similar to that of efflux pumps of the major facilitator superfamily.